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KMID : 0380420010250040213
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 4 p.213 ~ p.219
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Rapid Detection of Enterococcus Species by Polymerase Chain Reaction(PCR) and Prevalence in Livestock
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강현미/Kang, Hyun Mi
정병열/문진산/이희수/장금찬/김종만/정충일/Jung, Byeong Yeal/Moon, Jin San/Lee, Hee Soo/Jang, Gum Chan/Kim, Jong Man/Chung, Choong Il
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Abstract
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For the rapid diagnosis of Enterococcus species, we established PCR method with primer, Ent 1 and Ent 2. The 112 bp DNA fragment was amplified among most of enterococci isolates, indicating the specificity of PCR. Prevalence of Enterococcus spp. was investigated from mastitis milk and various livestock. Major enterococci from mastitis milk were E. faecalis (50.5%) and E. faecium (34.5%). Distributions of Enterococcus spp. from livestock samples were E. faecium (42.6%), E. faecalis (17.4%), E. hirae (17.1%), E. casseliflavus (14.4%), E. gallinarum (5.6%), E. durans (2.6%) and E. avium (0.3%). Among enterococci from feces of dairy cow, E. casseliflavus (44.0%) was found to be the most dominant strain following by E. hirae (21.3%). In native cattle (Hanwoo), E. hirae (38.5%) was the dominant strain, following by E. faecium (26.6%), E. faecalis (16.5%), E. durans (7.3%), E. casseliflavus (6.4%), E. gallinarum (3.7%), and E. avium(0.9%). Isolates from porcine showed the distribution of E. faecium(25.0%) and E. casseliflavus (75.0%). In chicken, prevalence of enterococci was E. faecium(68.1%), E. faecalis (27.1%), and E. gallinarum(4.9%).
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